| Rank | City | 1990 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Milwaukee | 628,300 | -0.1% |
| 2 | Madison | 190,816 | 1.1% |
| 3 | Green Bay | 96,697 | – |
| 4 | Racine | 84,420 | – |
| 5 | Kenosha | 81,030 | – |
| 6 | Appleton | 66,137 | – |
| 7 | West Allis | 63,205 | – |
| 8 | Waukesha | 57,450 | – |
| 9 | Eau Claire | 57,316 | – |
| 10 | Oshkosh | 56,774 | – |
| 11 | Janesville | 52,733 | – |
| 12 | La Crosse | 51,544 | – |
| 13 | Sheboygan | 49,718 | – |
| 14 | Wauwatosa | 49,341 | – |
| 15 | Fond du Lac | 38,690 | – |
| 16 | Wausau | 37,376 | – |
| 17 | Beloit | 36,085 | – |
| 18 | Brookfield | 35,156 | – |
| 19 | New Berlin | 33,504 | – |
| 20 | Greenfield | 33,337 | – |
| 21 | Manitowoc | 32,644 | – |
| 22 | Superior | 27,286 | – |
| 23 | Menomonee Falls | 26,836 | – |
| 24 | West Bend | 24,615 | – |
| 25 | Stevens Point | 23,994 | – |
| 26 | Neenah | 23,442 | – |
| 27 | Franklin | 21,855 | – |
| 28 | South Milwaukee | 20,980 | – |
| 29 | Oak Creek | 19,492 | – |
| 30 | Marshfield | 19,259 | – |
| 31 | Watertown | 19,225 | – |
| 32 | Mequon | 19,000 | – |
| 33 | Cudahy | 18,659 | – |
| 34 | Wisconsin Rapids | 18,489 | – |
| 35 | Muskego | 16,811 | – |
| 36 | De Pere | 16,687 | – |
| 37 | Ashwaubenon | 16,407 | – |
| 38 | Fitchburg | 15,952 | – |
| 39 | Sun Prairie | 15,473 | – |
| 40 | Greendale | 15,099 | – |
| 41 | Menasha | 14,703 | – |
| 42 | Allouez | 14,431 | – |
| 43 | Beaver Dam | 14,287 | – |
| 44 | Whitefish Bay | 14,279 | – |
| 45 | Shorewood | 14,113 | – |
| 46 | Glendale | 13,981 | – |
| 47 | Middleton | 13,852 | – |
| 48 | Menomonie | 13,841 | – |
| 49 | Germantown | 13,662 | – |
| 50 | Two Rivers | 13,152 | – |
| 51 | Chippewa Falls | 12,982 | – |
| 52 | Whitewater | 12,795 | – |
| 53 | Kaukauna | 12,416 | – |
| 54 | Onalaska | 12,247 | – |
| 55 | Brown Deer | 12,185 | – |
| 56 | Marinette | 11,860 | – |
| 57 | Pleasant Prairie | 11,750 | – |
| 58 | River Falls | 11,152 | – |
| 59 | Oconomowoc | 11,035 | – |
| 60 | Fort Atkinson | 10,278 | – |
| 61 | Monroe | 10,272 | – |
| 62 | Cedarburg | 10,154 | – |
| 63 | Howard | 9,983 | – |
| 64 | Merrill | 9,860 | – |
| 65 | Platteville | 9,837 | – |
| 66 | Grafton | 9,641 | – |
| 67 | Weston | 9,471 | – |
| 68 | Little Chute | 9,468 | – |
| 69 | Port Washington | 9,436 | – |
| 70 | St. Francis | 9,254 | – |
| 71 | Baraboo | 9,239 | – |
| 72 | Sturgeon Bay | 9,202 | – |
| 73 | Burlington | 8,984 | – |
| 74 | Waupun | 8,980 | – |
| 75 | Stoughton | 8,849 | – |
| 76 | Ashland | 8,744 | – |
| 77 | Monona | 8,661 | – |
| 78 | Portage | 8,644 | – |
| 79 | Rice Lake | 8,339 | – |
| 80 | Antigo | 8,334 | – |
| 81 | Hartford | 8,312 | – |
| 82 | Plover | 8,175 | – |
| 83 | Sparta | 7,937 | – |
| 84 | Rhinelander | 7,872 | – |
| 85 | Shawano | 7,825 | – |
| 86 | Tomah | 7,704 | – |
| 87 | Hales Corners | 7,652 | – |
| 88 | Fox Point | 7,236 | – |
| 89 | Ripon | 7,213 | – |
| 90 | Hartland | 6,952 | – |
| 91 | Plymouth | 6,949 | – |
| 92 | New London | 6,740 | – |
| 93 | Hudson | 6,437 | – |
| 94 | Jefferson | 6,371 | – |
| 95 | Lake Geneva | 6,330 | – |
| 96 | Elm Grove | 6,278 | – |
| 97 | Delavan | 6,180 | – |
| 98 | Sheboygan Falls | 6,127 | – |
| 99 | Waunakee | 5,963 | – |
| 100 | Reedsburg | 5,917 | – |
In 1990, the largest city in Wisconsin was Milwaukee, with a population of 628,300. The second largest city is Madison, with a population of 190,816.
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