| Rank | City | 1850 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Boston | 136,881 | 3.9% |
| 2 | Lowell | 33,383 | 4.8% |
| 3 | Salem | 20,264 | 3.0% |
| 4 | Roxbury | 18,364 | 7.3% |
| 5 | Charlestown | 17,216 | 4.1% |
| 6 | Worcester | 17,049 | 8.6% |
| 7 | New Bedford | 16,443 | 3.1% |
| 8 | Cambridge | 15,215 | 6.1% |
| 9 | Lynn | 14,257 | 4.3% |
| 10 | Springfield | 11,766 | 0.7% |
| 11 | Fall River | 11,524 | 5.5% |
| 12 | Taunton | 10,441 | 3.2% |
| 13 | Newburyport | 9,572 | 2.9% |
| 14 | Nantucket | 8,452 | -0.6% |
| 15 | Chicopee | 8,291 | – |
| 16 | Lawrence | 8,282 | – |
| 17 | Danvers | 8,109 | 4.9% |
| 18 | Dorchester | 7,969 | 5.0% |
| 19 | Gloucester | 7,786 | 2.1% |
In 1850, the largest city in Massachusetts was Boston, with a population of 136,881. The second largest city is Lowell, with a population of 33,383.
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