| Rank | City | 1990 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Jacksonville | 635,042 | 1.6% |
| 2 | Miami | 359,790 | 0.4% |
| 3 | Tampa | 280,826 | 0.3% |
| 4 | St. Petersburg | 240,368 | 0.1% |
| 5 | Hialeah | 187,905 | – |
| 6 | Orlando | 163,435 | – |
| 7 | Fort Lauderdale | 148,779 | – |
| 8 | Tallahassee | 125,448 | – |
| 9 | Hollywood | 122,793 | – |
| 10 | Clearwater | 97,217 | – |
| 11 | Gainesville | 92,602 | – |
| 12 | Miami Beach | 92,296 | – |
| 13 | Coral Springs | 79,137 | – |
| 14 | Cape Coral | 75,172 | – |
| 15 | Pompano Beach | 72,375 | – |
| 16 | Lakeland | 71,043 | – |
| 17 | West Palm Beach | 66,837 | – |
| 18 | Largo | 66,596 | – |
| 19 | Sunrise | 66,212 | – |
| 20 | Plantation | 66,116 | – |
| 21 | Pembroke Pines | 65,454 | – |
| 22 | Boca Raton | 62,866 | – |
| 23 | Palm Bay | 62,664 | – |
| 24 | Daytona Beach | 62,622 | – |
| 25 | Melbourne | 60,450 | – |
| 26 | Pensacola | 59,268 | – |
| 27 | Port St. Lucie | 55,759 | – |
| 28 | Davie | 51,515 | – |
| 29 | Sarasota | 51,041 | – |
| 30 | North Miami | 50,156 | – |
| 31 | Deltona | 49,145 | – |
| 32 | Lauderhill | 49,067 | – |
| 33 | Delray Beach | 47,748 | – |
| 34 | Deerfield Beach | 47,162 | – |
| 35 | Boynton Beach | 47,047 | – |
| 36 | Fort Myers | 44,645 | – |
| 37 | Tamarac | 44,469 | – |
| 38 | Pinellas Park | 43,968 | – |
| 39 | Bradenton | 43,665 | – |
| 40 | Margate | 43,179 | – |
| 41 | Ocala | 42,773 | – |
| 42 | Coral Gables | 41,428 | – |
| 43 | Miramar | 41,229 | – |
| 44 | Titusville | 39,619 | – |
| 45 | Fort Pierce | 37,519 | – |
| 46 | Port Orange | 36,933 | – |
| 47 | Altamonte Springs | 35,160 | – |
| 48 | Dunedin | 35,053 | – |
| 49 | Panama City | 35,039 | – |
| 50 | North Miami Beach | 35,030 | – |
| 51 | Sanford | 32,528 | – |
| 52 | Ormond Beach | 31,460 | – |
| 53 | Kissimmee | 30,648 | – |
| 54 | Hallandale Beach | 30,116 | – |
| 55 | Lake Worth | 28,840 | – |
| 56 | Homestead | 28,387 | – |
| 57 | Jupiter | 28,055 | – |
| 58 | Lauderdale Lakes | 27,914 | – |
| 59 | Riviera Beach | 27,362 | – |
| 60 | Coconut Creek | 27,241 | – |
| 61 | North Lauderdale | 26,812 | – |
| 62 | Winter Haven | 26,426 | – |
| 63 | Oakland Park | 25,645 | – |
| 64 | Key West | 24,940 | – |
| 65 | Winter Park | 24,886 | – |
| 66 | Palm Beach Gardens | 23,925 | – |
| 67 | Plant City | 22,859 | – |
| 68 | Winter Springs | 22,285 | – |
| 69 | Wellington | 21,754 | – |
| 70 | Fort Walton Beach | 21,258 | – |
| 71 | Casselberry | 21,196 | – |
| 72 | Cooper City | 20,211 | – |
| 73 | Naples | 19,871 | – |
| 74 | Greenacres | 19,457 | – |
| 75 | Tarpon Springs | 18,937 | – |
| 76 | Temple Terrace | 18,204 | – |
| 77 | Jacksonville Beach | 18,112 | – |
| 78 | Dania | 17,946 | – |
| 79 | Pinecrest | 17,939 | – |
| 80 | Venice | 17,523 | – |
| 81 | Cocoa | 17,491 | – |
| 82 | Vero Beach | 17,370 | – |
| 83 | New Smyrna Beach | 17,167 | – |
| 84 | De Land | 17,040 | – |
| 85 | Safety Harbor | 16,438 | – |
| 86 | Rockledge | 16,134 | – |
| 87 | Belle Glade | 15,853 | – |
| 88 | Royal Palm Beach | 15,612 | – |
| 89 | Edgewater | 15,541 | – |
| 90 | Bartow | 15,537 | – |
| 91 | Aventura | 15,384 | – |
| 92 | Opa-locka | 15,273 | – |
| 93 | Leesburg | 15,104 | – |
| 94 | Apopka | 15,082 | – |
| 95 | New Port Richey | 14,335 | – |
| 96 | Sweetwater | 13,889 | – |
| 97 | Miami Springs | 13,282 | – |
| 98 | Eustis | 13,186 | – |
| 99 | Ocoee | 13,146 | – |
| 100 | Longwood | 12,996 | – |
In 1990, the largest city in Florida was Jacksonville, with a population of 635,042. The second largest city is Miami, with a population of 359,790.
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